vivax isolates from representative epidemic-prone areas of South Korea are highly conserved.
Among the antibody-positive individuals, 13 (14.29%) had experienced malaria infection during the last 10 years. Of these samples, 91 (10.39%) showed a positive reaction with recombinant pGDH protein. The efficacy of recombinant pGDH protein in seroepidemiological studies was also evaluated by ELISA using serum samples collected from 876 inhabitants of Gyodong-myeon, Ganghwa County, Incheon Metropolitan City. The expressed recombinant protein had a molecular mass of approximately 55 kDa and showed 84.8% sensitivity (39/46 cases) and 97.2% specificity (35/36 cases) in an ELISA. vivax Bucheon 3 (KJ726751) and subcloned into the expression vector pET28b for transformation into Escherichia coli BL21(DE3)pLysS.
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The full ORF (amino acids 39–503), excluding the region before the intron, was cloned from isolate P. vivax strain Sal-I (GenBank accession No. The amino acid and nucleotide sequences were conserved among all the Korean isolates. vivax pGDH gene from the 20 Korean isolates showed that an open reading frame (ORF) of 1410 nucleotides encoded a deduced protein of 470 amino acids. Recombinant protein was prepared to determine the antigenicity of pGDH by enzyme-linked immunosorbent assay (ELISA). Genomic DNA was purified from blood samples of 20 patients and the pGDH gene of P. Variation in the pGDH gene among Korean isolates of Plasmodium vivax was analysed, and a recombinant pGDH protein was evaluated for use as antigens for the serodiagnosis of vivax malaria. Glutamate dehydrogenase of malaria parasites (pGDH) is widely used in rapid diagnostic tests for malaria.
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